Using high-performance liquid chromatography (HPLC) and 2,4-dinitrophenylhydrazine (2,4-DNPH) as a derivatizing reagent,\nan analytical method was developed for the quantitative determination of acetone in human blood.The determination was carried\nout at 365nm using an ultraviolet-visible (UV-Vis) diode array detector (DAD). For acetone as its 2,4-dinitrophenylhydrazone\nderivative, a good separation was achieved with a Thermo Acclaim C18 column (15 cm Ã?â?? 4.6mm Ã?â?? 3
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